Journal: Microorganisms

Article Title: Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription

doi: 10.3390/microorganisms14030564

Figure Lengend Snippet: Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in E. coli BL21(DE3), and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.

Article Snippet: BL21(DE3) Competent E. coli , C2527H , NEB Inc..

Techniques: Expressing, SDS Page, Plasmid Preparation, Control, Derivative Assay, SDS-Gel

Journal: Nature chemical biology

Article Title: Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors

doi: 10.1038/nchembio.1787

Figure Lengend Snippet: E. coli cells expressing MetSOx ( a ) or MetROx ( b ) were incubated with MetO for ~30 min, and then rinsed to remove the oxidant. Arrows indicate the addition (↓) of MetO and rinsing (↑) the cells for two representative experiments (Assay 1 and Assay 2). MetSOx ( c ) and MetROx ( d ) oxidized fractions calculated from (n = 3), using and , respectively, described in the “ online methods ” section.

Article Snippet: SoluBL21 TM E. coli (Gelantis, San Diego, CA) cells were transformed with an indicated expression vector and grown in Luria-Bertani containing ampicillin (50 μg.ml −1 ) at 37°C.

Techniques: Expressing, Incubation